Fig. 1: Summary of approach.
From: Neither alpha-synuclein fibril strain nor host murine genotype influences seeding efficacy
Two studies were conducted. Study 1, homozygous GBA1 D409V KI mice or WT mice were bilaterally injected with de novo αsyn PFFs or PBS into the olfactory bulb (OB) granule cell layer (GL). Study 2, PFFs were first generated with shaking and fragmentation by sonication in the presence of brain homogenates from idiopathic PD patients (iPD), or PD patients with heterozygous or homozygous GBA1 mutations. The resulting PFF polymorphs were then injected bilaterally into the OB of WT mice. Mice were sacrificed at 6 months post OB-injection and perfused with PBS followed by 4% PFA. Seeding and spread of αsyn pathology was determined with IHC detection of PSER129. Global PSER129 interactions were determined using BAR-PSER129. Partially created with BioRender.com.
