Fig. 4: Effect of various storage conditions on the seeding activities of skin-derived αSynD.
From: A skin-specific α-Synuclein seeding amplification assay for diagnosing Parkinson’s disease
a and b Streamlined SAA reactions seeded with skin homogenate from PD patients were analyzed under short-term storage conditions (0, 1, 3, and 7 days) at room temperature (RT) and 4 °C. c Streamlined SAA reactions seeded with skin homogenate from PD patients were analyzed after long-term storage (up to 3 years). d Streamlined SAA reactions seeded with skin homogenate from PD patients were analyzed after repeated freezing and thawing cycles (0, 2, 4, and 8 cycles). Numbers of positive replicates were calculated for each sample in percent. e and f Comparison of the lag phase and Fmax of the modified SAA between Nunc and polystyrene plates. Data are presented as mean ± SD of all PD patients analyzed in each group. One-way ANOVA followed by Tukey’s post-hoc-test was used in a–d, the two-tailed independent Student’s t-test was used in e and f, data are shown as mean ± SD. Data are statistically different from each other with *P < 0.05 and ***P < 0.001.
