Fig. 1: Examples of ELISA assays with monoclonal antibodies (mAbs). | npj Parkinson's Disease

Fig. 1: Examples of ELISA assays with monoclonal antibodies (mAbs).

From: Molecular properties and diagnostic potential of monoclonal antibodies targeting cytotoxic α-synuclein oligomers

Fig. 1

Indirect ELISA assays showing the extent of binding of the mAb 19-2C3-F10 to different fixed amounts of immobilized (a) α-syn monomers or (b) αSOs. c Summary of the data in panels a and b as a function of α-syn concentration in monomer units. d The apparent KD values obtained from fits of data in panels a and b are fitted to a simple binding isotherm, showing a steep increase in apparent affinity with the concentration of immobilized antigen. Estimation of stoichiometry based on intercept between initial linear increase and the plateau value leads to (e) a 1:1 molar ratio for the antibody 03-9H9, but (f) 1 mAb per αSO for the antibody 10-9C8-C1.

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