Fig. 2: Antibody-αSO affinities measured in different ways. | npj Parkinson's Disease

Fig. 2: Antibody-αSO affinities measured in different ways.

From: Molecular properties and diagnostic potential of monoclonal antibodies targeting cytotoxic α-synuclein oligomers

Fig. 2

a Values of IC50 measured for 27 different mAbs in ELISA competition assays using immobilized αSOs with monomeric α-syn present in the mobile phase. Errors from binding fits. b–e FIDA titration plots for binding of α-syn monomer and αSO to two different mAbs produced in this study (03-9H9 and 17-9D12-A1) and two commercial antibodies (MJF14 and pSer129). Errors from fits to individual FIDA curves. Associated KD values shown in Table 2 for in-house mAbs. For MJF14, the KD for αSOs and monomeric α-syn are 0.42 and 3.92 nM, respectively; for pSer129 the values are 0.455 and 3.0 µM. f The increased affinity of mAbs for αSOs relative to α-syn based on the ratio KDmonomer/ KDαSO. Data summarized in Table 2. g Microfluidic diffusional sizing (MDS) measurement of the binding of antibodies to α-syn fibrils, here exemplified with mAb 20-9E2-B8. Errors from fits to individual MDS curves. Data summarized in Table 3. Errors are standard errors of the mean based on triplicates.

Back to article page