Table 3 Data for binding of selected mAbs to α-syn in the fibrillated, oligomeric and monomeric state

From: Molecular properties and diagnostic potential of monoclonal antibodies targeting cytotoxic α-synuclein oligomers

mAb

Kdfibril (nM)a

nb

R2

Kdoligomer (nM)c

Kdmonomer (nM)c

10-9C8-C1

82.6 ± 105.2

2.33 ± 1.48

0.8753

6.1

97

17-9D12-A1

202.8 ± 238.3

1 d

0.8965

12

9.4

18-3A5-H2

3.82 ± 13.4

4.02 ± 0.93

0.7875

76

5767

19-2C3-F10

9.83 ± 17.1

1.845 ± 0.48

0.8473

4.2

312

20-9E2-B8

18.5 ± 15.9

5.58 ± 1.00

0.9509

(monomerizes)

3800

MJF14

36.3 ± 16.7

11 ± 2

0.9745

0.42

3.92

  1. aDetermined by microfluidic diffusional sizing.
  2. bα-syn monomers/binding site.
  3. cDetermined by FIDA.
  4. dThe model balances the concentration of binding sites available to the antibody with Kdfibril. Since the two values could compensate for each other (leading to a misleadingly high Kdfibril and a very low n and vice versa), n is restrained to be at least 1 monomer per binding site. The fit with 17-9D12-A1 was limited to this value.
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