Fig. 1: The evoked DA release and basal DA level are altered in the Synj1+/− neurons.
a Representative dLight1.3b sniffer cell response to a 20 Hz, 10 s field stimulation on a heatmap scale (left). Post hoc Immunolabeling of tyrosine hydroxylase (TH) and GFP determines the specificity of dLight response from proximal DAergic axons (right). Peripheral DAergic axons do not contribute significantly to the dLight response (data not shown). b Background subtracted ΔF/F0 dLight traces to sequential stimulations at 20 Hz from a representative neuron. c Representative ΔF/F0 dLight traces from Synj1+/+ and Synj1+/− littermate cultures at different stimulation lengths. d The ΔF/F0 dLight responses were normalized to the fluorescence at the end of stimulation and aligned to compare the decay curve (mean ± SEM) relative to baseline (F0, dotted line) for Synj1+/+ and Synj1+/− neurons at 20 Hz, 1 s (left), 2.5 s (middle) and 10 s (right) stimulations. e–h Summarized ΔF/F0 peak response (e), time to peak (f), decay time constant (g), and ΔF/F0 end fluorescence (h) in Synj1+/+ and Synj1+/− neurons from littermate cultures. **p < 0.01, Mann–Whitney test or Student’s t-test. Data from five batches of cultures. i Representative basal GRABDA1H fluorescence on a heatmap scale from littermate MB-sniffer cell cocultures. j Normalized GRABDA1H fluorescence from three batches of littermate cultures. ****p < 0.00001, Student’s t-test. k Analysis of DAergic axons in the Synj1+/+ or Synj1+/− cultures. Each data point is the analysis of one large view image (represented in the right panel). ≥3 fields were analyzed for each culture for a total of five batches of cultures. TH immunofluorescence was transformed into a binary image after background subtraction to calculate the TH axon area (right panels).
