Fig. 2: Increased DAT expression in Synj1+/− neurons. | npj Parkinson's Disease

Fig. 2: Increased DAT expression in Synj1+/− neurons.

From: Parkinson’s disease gene, Synaptojanin1, dysregulates the surface maintenance of the dopamine transporter

Fig. 2

a Representative coronal striatal slices from a Synj1+/+ mouse and a Synj1+/− mouse immunolabeled for Synj1 (red) and DAT (blue). Confocal images were captured using a lower and a higher magnification objective and presented in different scales. b Analysis of the optical density of DAT immunofluorescence at high magnification in the striatum from Synj1+/+ (n = 6 males + 4 females) and Synj1+/− (n = 6 males + 4 females) mice at 12–18 months. Each data point is an average value from one mouse. *p = 0.024, Student’s t-test with Welch correction for unequal variance. c Representative immunofluorescence images of cultured ventral midbrain neurons immunolabeled with anti-TH (green) and anti-DAT (red). White and blue boxed regions are axons and soma, respectively, which are magnified on the right panels. d and e Normalized immunofluorescence of DAT in the axons (d, by line selection) and soma (e, by area selection) of Synj1+/+ and Synj1+/− DA neurons. *p < 0.05, ****p < 0.0001. Mann–Whitney tests. Data from 2 to 3 batches of cultures.

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