Fig. 6: MCS and STN DBS have different effects on MC activities and motor behaviors in PD rats.
a, b MU (a, n = 133 MU segments from 7 rats) and SU (b, sorted from a) activities in MC before (Pre) and during DBS at STN (STN DBS) in PD rats. In (b), n = 147 SU clusters; 0 cluster vs 1 cluster vs >1 cluster, chi square test, χ2(2) = 1.33, p > 0.05 (left), and n = 91-129, 93-113, 83-109, and 90-117 SU clusters for quiescence/before DBS, active-moving/ before DBS, quiescence/during DBS, and active-moving/during DBS, respectively. *p < 0.05, **p < 0.01, ***p < 0.001, N.S., nonsignificant, Student’s t tests (lines) or simple main effects (lines)/main effects (brackets) from 2*2 ANOVA (see Methods). c, d SU activities in STN with MCS in PD rats (c, n = 48, 50, 53, and 50 SU clusters from 10 rats for quiescence/sham, active-moving/sham, quiescence/MCS, and active-moving/MCS groups, respectively) and normal rats (d, n = 79, 71, 108, and 106 SU clusters from 8 rats, respectively). e Continuous recordings of moving velocity in OFT over time in representative PD and normal rats. f Dose-dependent effects on behavioral parameters with STN DBS and MCS in PD rats (DBS: n = 6 rats, the current intensity with the maximal total moving distance without propulsive turning or restless turning is defined as 100% I; MCS: n = 6 rats, 100% I is the most enhancement dose without propulsive turning). The data of sham stimulation (sham) and of the first run in OFT from each normal rat (1st time in OFT) are also documented for comparison (from the same rats in Fig. 1i). The normal rats usually show “vigorous” onset of locomotor activities without lengthening the active moving episode for the first run. In contrast, STN DBS in PD rats induces significant changes in the duration of active moving episode, while MCS does not have similar effects. *p < 0.05, **p < 0.01, ***p < 0.001, N.S., nonsignificant, Friedman tests (data with 4 groups) or Mann-Whitney U tests (data with 2 groups). The illustrations in a and c are created in BioRender.
