Fig. 2: LPC induced α-Syn aggregation in dopaminergic neurons.

a–d Western blot experiments were conducted to assess α-Synuclein expression levels in MES23.5 dopaminergic neurons following LPC intervention at varying doses (5 μg/mL, 10 μg/mL, 15 μg/mL) of LPC (n = 3, oligomers: Control vs LPC5: p = 0.0074, Control vs LPC10: p = 0.0004, Control vs LPC15: p = 0.0004; monomer: Control vs LPC5: p = 0.0379, Control vs LPC10: p = 0.0001, Control vs LPC15: p < 0.0001). e–h Western blot analyses were performed to evaluate α-Synuclein expression in MES23.5 dopaminergic neurons post-LPC and MPP⁺ interventions (n = 3, oligomers: Control vs LPC: p = 0.0041, Control vs MPP⁺: p = 0.0007, Control vs LPC + MPP⁺: p = 0.0002, MPP⁺ vs LPC + MPP⁺: p = 0.0008; monomer: Control vs LPC: p < 0.0001, Control vs MPP⁺: p < 0.0001, Control vs LPC + MPP⁺: p < 0.0001, MPP+ vs LPC + MPP⁺: p < 0.0001; oligomers/ monomer: Control vs LPC: : p = 0.0293, Control vs MPP⁺: p < 0.0001, Control vs LPC + MPP⁺: p < 0.0001, MPP + vs LPC + MPP⁺: p = 0.0352). i Immunofluorescence co-labeling images showing TH and α-Synuclein in SNC dopaminergic neurons of C57BL/6 J mice post-LPC injection. j Quantification of α-Synuclein immunofluorescence intensity in TH-positive regions (n = 3, t = 17.86, p < 0.0001). k Representative α-Synuclein immunofluorescence images in MES23.5 dopaminergic neurons after LPC and MPP⁺ interventions. l Analysis of α-Synuclein immunofluorescence intensity in MES23.5 dopaminergic neurons (n = 6, Control vs LPC: p = 0.0003, Control vs MPP⁺: p < 0.0001, Control vs LPC + MPP⁺: p < 0.0001, MPP⁺ vs LPC + MPP⁺: p = 0.01).