Fig. 8: LPC impairs the autophagy mechanism of α-synuclein in dopaminergic neurons, exacerbating the accumulation of α-Syn. | npj Parkinson's Disease

Fig. 8: LPC impairs the autophagy mechanism of α-synuclein in dopaminergic neurons, exacerbating the accumulation of α-Syn.

From: Lysophosphatidylcholine promoting α-Synuclein aggregation in Parkinson’s disease: disrupting GCase glycosylation and lysosomal α-Synuclein degradation

Fig. 8

a Volcano plot illustrating the top 20 mRNAs identified for both up- and down-regulation. b, c GO and KEGG pathway analysis targeting differentially expressed genes. d–f Western blot (WB) experiments assessing the levels of the autophagy indicator P62 in MES23.5 dopaminergic neurons following intervention with varying doses of LPC (n = 3, Control vs LPC5: p < 0.0001, Control vs LPC10: p = 0.0001, Control vs LPC15: p = 0.1170, non-significant), and LC3B II levels (n = 3, Control vs LPC5: p < 0.0001, Control vs LPC10: p < 0.0001, Control vs LPC15: p < 0.0001). g, h Immunofluorescence co-labeling of α-Syn with P62 in MES23.5 dopaminergic neurons post-LPC intervention and co-localization analysis (n = 3, t = 10.4, p = 0.0005). i Statistics of α-Syn immunofluorescence intensity in MES23.5 dopaminergic neurons (n = 3, t = 19.74, p < 0.0001). j Statistics of P62 immunofluorescence intensity in MES23.5 dopaminergic neurons (n = 3, t = 4.301, p = 0.0157). k, l ImageJ line plot depicting the co-localization analysis of α-Syn and P62. (m, n) Immunofluorescence co-labeling of α-Syn and LC3B in MES23.5 dopaminergic neurons following LPC intervention and co-localization analysis (n = 3, t = 6.313, p = 0.0032). o Statistics of α-Syn immunofluorescence intensity in MES23.5 dopaminergic neurons (n = 3, t = 17.98, p < 0.0001). p Statistics of LC3B immunofluorescence intensity in MES23.5 dopaminergic neurons (n = 3, t = 18.35, p < 0.0001). q, r ImageJ line plot depicting the co-localization analysis of α-Syn and LC3B.

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