Fig. 1: A new amino acid-centric analysis approach for higher-resolution structural comparison with LiP-MS. | npj Parkinson's Disease

Fig. 1: A new amino acid-centric analysis approach for higher-resolution structural comparison with LiP-MS.

From: An approach to characterize mechanisms of action of anti-amyloidogenic compounds in vitro and in situ

Fig. 1

A Volcano plot comparing LiP peptide abundances generated upon limited proteolysis of α-Synuclein monomers and fibrils. B All detected and quantified LiP peptides and their corresponding scores (−log10(q-value) x |log2(fold change)|) were mapped along the α-Synuclein sequence. The scale reflects the score for every peptide. The more intense the red color, the higher the score. For all panels, not significant is shown in gray, not detected in yellow. C Structural fingerprint comparing α-Synuclein monomer and fibril after applying the classical LiP-MS data analysis pipeline. Significant regions (|log2FC| > 1, q-value < 0.05) in red. D Structural fingerprint comparing α-Synuclein monomer and fibril upon scoring changes per amino acid. The scale indicates the score per amino acid. The significance threshold of −log10(0.05) × |log2(2)| is shown in white, with red indicating higher scores. The more intense the red color, the higher the score. N-term N-terminus, NAC non-amyloid β component (aa 61–95), C-term C-terminus.

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