Fig. 2: Glucocerebrosidase activity in VMDA and cortical neurons of the different mutation groups.

ARepresentative images of the PFB-FDGlu fluorescence (white) in VMDA neurons at 45 min after addition of the substrate, with Hoechst (blue). B GCase activity index calculated for each cell line of the different mutation groups in VMDA neurons. C Representative images of the DQ red BSA fluorescence (magenta) in VMDA neurons at 90 min after addition of the substrate with Hoechst (Blue). D Intensity of DQ red BSA fluorescence per cell calculated for each cell line of the different mutation groups in VMDA neurons. E Representative images of the PFB-FDGlu fluorescence (white) in the cortical neurons at 45 min after addition of the substrate, with Hoechst (blue). F GCase activity index calculated for each cell line of the different mutation groups in cortical neurons. G GCase activity index normalised with the protein expression level of GBA for each cell line of the different mutation groups. H Representative images of the DQ red BSA fluorescence (white) in cortical neurons at 90 min after addition of the substrate with Hoechst (Blue). I Intensity of DQ red BSA fluorescence per cell calculated for each cell line of the different mutation groups in cortical neurons. The scale bar in all images is 200 µm. Image quantification graphs show estimated marginal mean ± SEM. Each datapoint on the graph represents the mean value for each cell line derived from n = 3 biological replications (each performed in at least duplicate).