Fig. 5: Alpha-synuclein staining shows distinct pathology in PRKN mutation dopamine neurons. | npj Parkinson's Disease

Fig. 5: Alpha-synuclein staining shows distinct pathology in PRKN mutation dopamine neurons.

From: Dopamine and cortical neurons with different Parkinsonian mutations show variation in lysosomal and mitochondrial dysfunction

Fig. 5

A Representative images of VMDA neurons immunostained with total α-syn (magenta), TH (green) and DAPI (blue). B α-syn intensity per cell in the different mutation groups of VMDA neurons. C Number of α-syn spots per cell in the different mutation groups of cortical neurons. D Representative images of VMDA neurons immunostained with S129-phospho-α-syn (magenta), TH (green) and DAPI (blue). E P-α-syn intensity per cell in the different mutation groups of VMDA neurons. F number of p-α-syn spots per cell in the different mutation groups of VMDA neurons. G Representative images of cortical neurons immunostained with α-syn (magenta), MAP2 (green) and DAPI. H α-syn intensity per cell in the different mutation groups of cortical neurons. I Number of α-syn spots per cell in the different mutation groups of cortical neurons. J Representative images of cortical neurons immunostained with p-α-syn (magenta), MAP2 (green) and DAPI. K P-α-syn intensity per cell in the different mutation groups of cortical neurons. L Number of p-α-syn spots per cell in the different mutation groups of cortical neurons. The scale bar in all images is 200 µm. Image quantification graphs show estimated marginal mean ± SEM. Each datapoint on the graph represents the mean value for each cell line derived fromn = 3 biological replications (each performed in at least duplicate).

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