Fig. 5: Modulation of excitatory and inhibitory transporters and microglia activation after 6-OHDA injection.

A VGAT puncta-rings analysis. Magnified view of the primary motor cortex layer II/III stained with anti-VGAT for control. Scale bar: 20 µm. B Interhemispheric analysis of anti-VGAT fluorescence mean values of puncta-rings around cell bodies in control (n = 8) and 6-OHDA group (n = 13), calculated in perisomatic puncta-rings intensity around cell bodies. C VGLUT1 puncta-rings analysis in layer II/III of control and 6-OHDA groups. Magnified view of the primary motor cortex stained with anti-VGLUT1. Scale bar: 20 µm. D Interhemispheric analysis of anti-VGLUT1 fluorescence mean values of puncta-rings in control (n = 8) and 6-OHDA group (n = 13). E VGLUT2 puncta-rings analysis in layer II/III of control and 6-OHDA groups. Magnified view of the primary motor cortex stained with anti-VGLUT2. Scale bar: 20 µm. F Interhemispheric analysis of anti-VGLUT2 fluorescence mean values of puncta-rings in control (n = 8) and 6-OHDA group (n = 13). G–I Phagocytosis analysis of Iba1-positive cells in the primary motor cortex layer II/III. G Representative tridimensional surface analysis of Iba1, CD68 and VGLUT1 markers in the primary motor cortex stained with anti-Iba1, anti-CD68 and anti-VGLUT1. Scale bar: 7 µm. H Interhemispheric analysis of phagosome volume (CD68⁺) inside Iba1⁺ cells. n = 60 cells for each dataset and CNT animals n = 4 vs. 6-OHDA animals n = 5 were analyzed. I Interhemispheric analysis of VGLUT1 engulfment inside CD68 volume, normalized on the total amount of VGLUT1 in the field. n = 60 cells for each dataset and CNT animals n = 4 vs. 6-OHDA animals n = 5 were analyzed. Data are expressed as mean ± Standard Error of Mean. *p ≤ 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. See also Supplementary Fig. 5 and Supplementary Table 4.