Fig. 4: Lrrk2 G2019S mutation leads to increased colon morphological damage after C. rodentium infection.
Male and female Lrrk2 G2019S and WT mice (male-to-female ratio = 0.43) were infected once with ~1 × 109 CFU of C. rodentium. Colons were harvested 7 days after infection, fixed in 10% formalin for 24 h, washed in 70% ethanol, and diaphanized in xylene before embedding in paraffin. Sections were stained with Hematoxylin and Eosin (H&E) and scored for histopathology. A Total histopathology score. B H&E representative images. Data are presented as mean ± SD and analyzed by two-way ANOVA with Fisher’s LSD post-test. **p < 0.01. n = 7–11 mice per group. Two independent experiments were done and showed as a pool. White bars represent 100 µm. Symbols: blue star, edema; green arrow, inflammatory infiltrate; red arrow, erosion; yellow star, goblet cell loss. Please, also see Supplementary Fig. 8.
