Fig. 2: Shorter α-syn mPFFs show a higher seeding activity in primary neurons. | npj Parkinson's Disease

Fig. 2: Shorter α-syn mPFFs show a higher seeding activity in primary neurons.

From: Impact of α-synuclein fibril structure on seeding activity in experimental models of Parkinson’s disease

Fig. 2

A Representative images of α-syn aggregates in mouse primary hippocampal neurons 3 days after the addition of each mPFF, using an antibody against p-α-syn. Scale bars: 100 nm. B P-α-syn positive area divided by the DAPI-positive area to normalize for the number of cells. The average value for S30 was set to 1 on the vertical axis. S30 forms the strongest pathology. Each plotted dot represents the average from four independent well regions, and six independent experiments were analyzed. A one-way ANOVA with Tukey’s multiple-comparisons test is performed; *p < 0.05, **p < 0.0001. Data are expressed as the mean ± SEM. C Western blot analysis using a p-α-syn-specific antibody. p-α-syn pathology worsened in a time-dependent manner up to 30 min of sonication but decreased after 60 and 120 min of sonication (n = 3 for sonicated or unsonicated mPFFs, and n = 2 for monomer or negative control). D Densitometric analysis of (C). The total density of each sample is measured and normalized against actin levels. A one-way ANOVA with Tukey’s multiple-comparisons test is performed; *p < 0.05, **p < 0.005, ***p < 0.0001.

Back to article page