Fig. 3: Validation of the sensitivity of the α-syn-isSID assay. | npj Parkinson's Disease

Fig. 3: Validation of the sensitivity of the α-syn-isSID assay.

From: Novel in situ seeding immunodetection assay uncovers neuronal-driven alpha-synuclein seeding in Parkinson’s disease

Fig. 3

Representative images of independent experiments are shown. Electron microscopy (EM) analysis of α-syn PFFs (left). When α-syn-isSID is assayed on poly-D-lysine-coated coverslips spotted with α-syn PFFs, robust fluorescent signal is detected at 1 mg/mL, with reduced but still detectable signal at 1 µg/mL (right), representing the assay’s sensitivity (a). EM analysis of α-syn monomers (left). Correspondingly, no signal is observed when α-syn-isSID is applied to α-syn monomers (right), supporting the assay’s specificity for aggregated species (b). RT-QuIC assay confirms the seeding competence of α-syn PFFs and the lack of seeding activity of monomeric α-syn (c). Scale bar of the EM images is 1000 nm, and 100 µm for the α-syn-isSID images.

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