Fig. 7: EV-associated proteolysis of α-syn does not induce the formation of aggregated α-syn species in neurons.
From: Proteolytic activities of extracellular vesicles attenuate A-synuclein aggregation
Confocal microscopy images of primary cortical neurons labelled for aggregated α-syn (MJFR-14). Cells were treated at 5 DIV with hPFFs, hPFFs pre-incubated with EVs in the presence or absence of protease inhibitors, as well as with α-syn monomers pre-incubated with EVs in the presence or absence of protease inhibitors (PIs), for 96 h. Untreated cells, as well as cells treated only with EVs were used as controls. TUJ1 and DAPI were used to stain neurons and neuronal nuclei, respectively. Scale bar represents 20 μm.
