Fig. 8: Injection of EV-cleaved PFFs causes significantly reduced α-Syn pathology in WT mice, compared to non-cleaved PFFs, at 1 month post inoculation.
From: Proteolytic activities of extracellular vesicles attenuate A-synuclein aggregation
A Representative confocal images of the contralateral and ipsilateral striatum and SNpc of uncleaved PFF- (left panel) and EV-Cleaved PFF-injected (right panel) mice, 1-month post-injection, showing double immunostaining of phosphorylated-α-Syn (green) and tyrosine hydroxylase (TH, red). [(low magnification; 10x, scale bar 50 μm and high magnification; 63x, scale bar 20 μm)]. B Graph illustrating the estimated α-Syn pathology in the ipsilateral SNpc, at 1-month post-intrastriatal injections, expressed as % of double positive phospho-α-Syn/TH neurons, normalized to the total number of dopaminergic neurons (upper panel). Graph depicting the stereological counts of the nigral dopaminergic cell somata, estimated as the percentage of TH positive neurons of the ipsilateral side versus the ones found in the contralateral side (lower panel). (n = 4 animals per group). Data represent mean values ± SEM. Differences were estimated using nonparametric Mann-Whitney.
