Table 2 Binding parameters of VASH1-SVBP complex interaction with tyrosinated- and L-Dopa-enriched microtubules

From: L-Dopa-modified microtubules lead to synapse instability in cultured neurons: possible implications in Parkinson’s disease therapy

Experiment

Recombinant protein (concentration)

Microtubule

Residence time τ (s)

Binding frequency

(min-1.nM-1.µm-1)

a

deadVASH1-SVBP

(50 pM)

Tyr-MTs

2.2

72 ± 5

L-Dopa-MTs

1.5

130 ± 7

b

deadVASH1-SVBP

(25 pM)

Tyr-MTs

8.7

65 ± 7

L-Dopa-MTs

6.3

85 ± 9

c

deadVASH1-SVBP

(25 pM)

Tyr-MTs

9.5

41 ± 4

L-Dopa-MTs

3.6

73 ± 8

  1. Resident time and binding frequency results corresponding to experiments performed on different days, using different protein preparations. At least 15 microtubules per condition were analyzed. (a) Related to Supplementary Fig. 2. (b, c) Related to Fig. 2.
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