Fig. 2: MGO-glycated aSyn induces pS129 positive inclusions in primary neurons.
From: Glycation of alpha-synuclein enhances aggregation and neuroinflammatory responses
A Primary rat cortical neurons were incubated for 20 days with 50 nM aSyn species. Following wash and fixation, cells were immunostained with Tuj1 and pS129, representative images from N = 3 (Scale bar 50 μm). B After sequentially extracting the cells with TritonX-100 and SDS containing buffer, extracts were analyzed using immunoblots with Syn1 and β-actin as loading control. C The ratio of aSyn and β-actin signal was determined (n = 4, mean ± SD). D The ratio of aSyn HMWs (above ~55 kDa) versus β-actin was quantified (n = 3, mean ± SD). E Cytotoxicity under different conditions of treatment was measured by the activity of released LDH and is displayed as per cent cytotoxicity (n = 5).
