Fig. 1: Upregulation of IFI16 expression during trilineage specification.
From: IFI16 promotes human embryonic stem cell trilineage specification through interaction with p53
a–c Quantitative PCR analysis of IFI16 mRNA levels in H9 cells and differentiated trilineage (a, endoderm; b, mesoderm; c, ectoderm; n = 4 in each group) for indicated periods of time. The relative mRNA levels of IFI16 in indicated time courses were calculated relatively to which at 0 h. d–f Representative immunoblots of IFI16, AIM2, and cGAS from H9 cells and differentiated trilineage (d, endoderm; e, mesoderm; f, ectoderm) for indicated periods of time. β-actin serves as a loading control. g Representative immunofluorescence images staining with antibodies against IFI16, AIM2, and cGAS in H9 cells and the differentiated trilineage. DAPI serves as a nucleus indicator. Scale bar, 200 µM. ENDO, endoderm; MESO, mesoderm; ECTO, ectoderm. All data were presented as mean ± SEM. Comparisons between groups for statistical significance were performed with one-way ANOVA with Tukey’s post hoc test. **P < 0.01, ***P < 0.001 versus H9.
