Fig. 2: JNK activation is responsible for IFI16 upregulation during trilineage specification.

a–c Representative immunoblots of total lysates from H9 cells and differentiated trilineage (a, endoderm; b, mesoderm; c, ectoderm) for indicated periods of time and probed with the antibodies for p-JNK, JNK, p-ERK, ERK, for p-p38 and p38. d–f, quantitative PCR analysis of IFI16 mRNA levels in H9 cells and differentiated trilineage after incubation with SP600125 (10 µM, n = 4, (d), U0126 (10 µM, n = 3, (e), and SB203580 (10 µM, n = 4, f The relative mRNA level of IFI16 was calculated relatively to which in H9 cells. g, h quantitative PCR examination of IFI16 promoter (n = 4), IFI16 3’UTR (n = 4), AIM2 promoter (n = 3), and cGAS promoter (n = 3) levels pulled-down by c-Jun antibodies in H9 cells and differentiated trilineage. The relative c-Jun associated DNA level was calculated relatively to which in H9 cells. ENDO, endoderm; MESO, mesoderm; ECTO, ectoderm. NC, negative control. All data were presented as mean ± SEM. Comparisons between groups for statistical significance were performed with one-way ANOVA with Tukey’s post hoc test (g, h) or two-way ANOVA with Bonferroni post hoc test (d–f). *P < 0.05, **P < 0.01, ***P < 0.001 versus 0 h, NC or H9.