Fig. 5: IFI16 interacts with p53 during trilineage specification.
From: IFI16 promotes human embryonic stem cell trilineage specification through interaction with p53
a–c Representative immunoblots of p53 from H9 cells and differentiated trilineage (a, endoderm; b, mesoderm; c, ectoderm) infected with sh1865 or shNC. d–f Statistics for a–c respectively, n = 4 for each group. The relative protein level of p53 was calculated relatively to which in H9-shNC group. g–h lysates of H9 cells and differentiated trilineage on Day 2 precipitated with p53 (g) and IFI16 (h) antibodies, and immunoblotted with indicated antibodies. β-actin serves as a loading control. i PLA analysis of IFI16-p53 interaction in H9 cells and differentiated trilineage on Day 2. DAPI serves as a nucleus indicator. Dashed boxes indicate the zoom in reagions in each image. Scale bar, 50 µM. ENDO, endoderm; MESO, mesoderm; ECTO, ectoderm. NC, negative control. All data were presented as mean ± SEM. Comparisons between groups for statistical significance were performed with two-way ANOVA with Bonferroni post hoc test (d–f). *P < 0.05, **P < 0.01, ***P < 0.001 versus H9-NC Day 0.
