Fig. 2: The extracellular matrix composition of developed scaffolds was assessed. | npj Regenerative Medicine

Fig. 2: The extracellular matrix composition of developed scaffolds was assessed.

From: Towards a bioengineered uterus: bioactive sheep uterus scaffolds are effectively recellularized by enzymatic preconditioning

Fig. 2

A Russell-Movat pentachrome stains collagen and reticular fibers (yellow), nuclei and elastic fibers (black), sulfated glycosaminoglycans (blue), muscle tissue (red), and fibrin intense red. Stained from scaffolds revealed that the matrix metalloproteinase 2 and 9 (MMPs) treatment resulted in a more porous scaffold and that elastic fibers became more distinct. Scale bars = 100 µm. B Each decellularization method resulted in scaffolds with very small amounts of remaining donor DNA, and that the MMPs were able to reduce this even more in protocol (P) 2 derived scaffolds. C The total protein content and D the hydroxyproline content were unaffected by the MMPs treatment while E the collagen fiber thickness was significantly reduced. Graphs show box plot with each sample value, median ± IQR, and range.

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