Fig. 5: Pluripotent cells used for the recellularization. | npj Regenerative Medicine

Fig. 5: Pluripotent cells used for the recellularization.

From: Towards a bioengineered uterus: bioactive sheep uterus scaffolds are effectively recellularized by enzymatic preconditioning

Fig. 5

Heterogeneous sheep fetal stem cells (SF-SCs) were isolated from a 6–8-week-old sheep fetus (A). These cells were expanded in vitro and their pluripotency was demonstrated after their differentiation into a chondrogenic lineage (alcian blue positive clusters; B), an osteogenic lineage positive for dentin matrix acidic phosphoprotein 1 (DMP1) and receptor activator of nuclear factor κ B (RANK; C), and a myogenic lineage positive for smooth muscle cell actin (SMA) and myoblast determination protein 1 (MyoD1; D). Scale bars; 5 cm (A), and 100 μm (B–D).

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