Fig. 4: Effects of rhACE2 patch on neonatal rat cardiomyocytes (NRCMs) and cardiac fibroblasts in vitro. | npj Regenerative Medicine

Fig. 4: Effects of rhACE2 patch on neonatal rat cardiomyocytes (NRCMs) and cardiac fibroblasts in vitro.

From: Myocardial fibrosis reversion via rhACE2-electrospun fibrous patch for ventricular remodeling prevention

Fig. 4

a Representative immunofluorescence staining of NRCMs exposed to 6 h hypoxia and cocultured with different patches was observed with α-actinin antibody (green), DAPI (blue), and TUNEL (red). Scale bars represented 50 μm. b Statistical analysis of TUNEL-positive cell counts per 100 cells. n = 5/group. c Representative flow cytometric Annexin V FITC-A vs Propidium Iodide-A quad plots of NRCMs after 6 h hypoxia treatment. d The percentage of Annexin V−/PI− cell were summarized. n = 3/group. e Representative wound healing assay image of cardiac fibroblasts cocultured with different patches after 24 h. f Statistical analysis of healed wound area difference between two groups. n = 5/group. Data were represented as the mean ± SEM. Two group comparisons were analyzed by the unpaired Student’s t-test; **P < 0.01, ****P < 0.0001.

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