Fig. 2: Quantitative analysis of LB opacification.
From: Modeling congenital cataract in vitro using patient-specific induced pluripotent stem cells
a Left panel: Schematic illustrating the method of measuring the fractional area of transparent regions (TR%) in the LBs. Right panel: Representative image of a LB showing the method of fractional area measurement. L: LB; TR: transparent region; SC: supporting cells. The dashed red lines indicate the borders of the LBs. The blue dashed lines indicate the borders of transparent regions. Scale bars: 200 μm. b Representative images and the TR% of the normal, CRYBB2-, and CRYGD-mutated LBs. The dashed red lines indicate the borders of LBs. The blue dashed lines indicate the borders of transparent regions. Scale bars: 200 μm. c TR% analysis of the normal, CRYBB2-, and CRYGD-mutated LBs. The data are presented as mean ± SD. **p < 0.01 versus normal, ##p < 0.01 versus the indicated group.
