Fig. 3: Expression characteristics of the CRYGD- and CRYBB2-mutated LBs.
From: Modeling congenital cataract in vitro using patient-specific induced pluripotent stem cells
a Immunofluorescence analysis of the human mature lens-specific markers on D25. Scale bar: 200 μm. b qRT-PCR analysis of the human mature lens-specific markers on D25. *p < 0.05, **p < 0.01 versus normal. c Representative images of αA-crystallin aggregates in the CRYGD- and CRYBB2-mutated LBs on D25. Cells with inhomogeneous punctate staining (i.e., aggregates) are indicated by white arrows. Scale bars: 100 μm (upper panels), 25 μm (lower panels). d, e Quantitative analysis of the percentage of cells with protein aggregates (d) and the mean grayscales of the cells with aggregates (e) in the normal and the patient-specific LBs. At least three fields (250×) from each group were randomly chosen for analysis. **p < 0.01 versus normal, ##p < 0.01 CRYBB2 versus CRYGD. f SDS-PAGE gel electrophoresis of the soluble and insoluble proportions of the protein of the normal and patient-specific LBs on D25. The gels derived from the same experiment and were processed in parallel. T total protein, S soluble protein, P insoluble protein. g Quantitative analysis of the ratio of soluble and insoluble protein. **p < 0.01 versus normal. The data are presented as the mean ± SD of experiments from at least three parallel samples per group.
