Fig. 4: Structural analysis of the normal and the CRYGD-mutated LBs.
From: Modeling congenital cataract in vitro using patient-specific induced pluripotent stem cells
a–d DiOC6 staining of the normal (a) and CRYGD-mutated LBs (b–d) on D25. The white arrow indicates cord-like structures in the CRYGD-mutated LBs. Scale bar: (a–c) 100 μm, (d) 25 μm. e Immunofluorescence analysis of collagen IV in the normal and CRYGD-mutated LBs on D25. Scale bar: 100 μm. f Immunofluorescence analysis of the lens epithelial marker E-cadherin in the normal and CRYGD-mutated LBs on D10, D18, and D25. Scale bar: 100 μm. g–p TEM images of the normal and CRYGD-mutated LBs on D25. Lens capsules (asterisks in g and l), lens epithelial cells (arrows in g, h, i, l, and m), and differentiating lens fiber cells (j, k, o, and p, arrow heads in i and n) with degenerating nuclei (arrow in p), degenerating organelles (arrow in k), and autophagosomes (arrows in j and o). Scale bars: (g, l, and n) 1 μm, (h, i, and m) 2 μm, (j, k, o, and p) 0.5 μm.
