Fig. 4: Myocardial ischemic injury response in Acomys and Mus.
From: Adult spiny mice (Acomys) exhibit endogenous cardiac recovery in response to myocardial infarction
a Representative M-mode echocardiography images of Mus-C57BL6 (left), Mus-CFW (middle), and Acomys (right) at baseline (upper images) and day 50 after MI (lower images). Delta (Δ) ejection fraction (EF) (b), Δ left ventricular end-diastolic diameter (LVEDD) (c), Δ left ventricular end-systolic diameter (LVESD) (d), Δ left ventricular end-diastolic volume (LVEDV) (e), and Δ left ventricular end-systolic volume (LVESV) (f) after injury, calculated as difference between each time point and baseline value in the same animal (N = 16–45 Mus-C57BL6, 10–20 Mus-CFW and 8–11 Acomys) (Values are means ± S.E.M, *P < 0.05 by Mixed-effects ANOVA, compared to *Mus-C57BL6 or #Mus-CFW). g Quantitation of heart weight (HW) normalized by body weight (BW) for Mus-C57BL6 (N = 2–26 animals), Mus-CFW (N = 3–11 animals) and Acomys (N = 2–13 animals) at baseline and various timepoints after MI suggesting maintenance of HW/BW ratio in Acomys and progressive increase in Mus (Values are means ± S.E.M, P < 0.05 by two-way independent ANOVA, compared to *Mus-C57BL6 or #Mus-CFW). h Quantitation of wet-dry lung weight normalized by body weight of Mus-C57BL6 (2–26 animals), Mus-CFW (3–11 animals) and Acomys (1–13 animals) at various timepoints after MI suggesting maintenance of wet-dry lung weight/body weight ratio in Acomys and progressive increase in Mus (Values are means ± S.E.M, *P < 0.05 by two-way independent ANOVA, compared to *Mus-C57BL6 or #Mus-CFW). i Kaplan–Meier analysis depicting mortality after MI and showing significantly lower mortality in Acomys compared with Mus (Gehan–Bareslow–Willcoxon test, P < 0.05). j Representative cardiac magnetic resonance (CMR) images showing comparably medium-sized infarct area in both species at 3 days post MI (arrows show the boundaries of late gadolinium enhancement/injury). The progression of infarct area was significantly slower in Acomys compared to Mus at 14 days after MI (N = 9–10 animals per group, t = 3.28, *P < 0.05 by paired T-test).
