Fig. 3: Scar organization is different in Acomys and Mus hearts.
From: Ischemic tolerance and cardiac repair in the spiny mouse (Acomys)
a Hydroxyproline assay from whole heart ventricle homogenates. b TNBSA assay from whole heart ventricle homogenates, normalized to total collagen content (hydroxyproline assay) and sham-control. c Polarized light imaging of injured hearts to analyze collagen fiber thickness. Left panel are representative images of the scar center (14 dpi) with the corresponding quantification on the right. d N-hydroxysuccinimide ester labeling and whole-mount imaging of injured hearts (14 dpi). Scale bar = 200 µm (overview) and 50 µm (inlet). e Waviness of individual collagen fibers, quantified from (d). a, b, c, e Two-way mixed ANOVA followed by Bonferroni post hoc test. For all comparisons: * is significant compared to Acomys-sham, # compared to Mus-sham, $ is significant in an inter-species comparison of the same time point (e.g. 100 days to 100 days). * is p < 0.05, ** is p < 0.01, and *** is p < 0.001. Box plots represent the median, interquartile range (IQR), minimum (25th percentile – 1.5 × IQR), and maximum (75th percentile – 1.5 × IQR).
