Table 1 Antibody and fluorescent dye list.
From: Ischemic tolerance and cardiac repair in the spiny mouse (Acomys)
Company | Catalog number | Working dilution | Antigen retrieval | |
|---|---|---|---|---|
Primary antibodies | ||||
α-SMA-647 | Novus Biologicals | NBP2-34522AF647 | 1:100 | No |
Phospho-Histone H3 | Novus Biologicals | NB600-1168 | 1:200 | Yes |
α-SMA | Abcam | ab5694 | 1:200 | No |
PDGFRβ | Abcam | ab32570 | 1:200 | Yes (but not required) |
ERG | Abcam | ab92513 | 1:200 | Yes |
MLC-2v | Synaptic Systems | 310 003 | 1:200 | Yes (but not required) |
DDAH2 | Elabscience | E-AB-10938 | 1:200 | No |
Secondary antibodies | ||||
Goat anti-Rabbit IgG (H + L)−488 | Invitrogen | A-11034 | 1:500 | NA |
Donkey anti-Rabbit IgG (H + L)−568 | Invitrogen | A-10042 | 1:500 | NA |
Goat anti-Rabbit IgG (H + L)−647 | Invitrogen | A-21245 | 1:500 | NA |
Goat anti-Rat IgG (H + L)−594 | Invitrogen | A-11007 | 1:500 | NA |
Goat anti-Rat IgG (H + L)−647 | Invitrogen | A-21247 | 1:500 | NA |
Fluorescent dyes | ||||
Isolectin GS-IB4-568 | Invitrogen | I21412 | 1:200 | NA |
Wheat Germ Agglutinin-488 | Invitrogen | W11261 | 1:200 | NA |
Hoechst 33342 | Invitrogen | H3570 | 1:2000 | NA |
Phalloidin-TRITC | Sigma Aldrich | P1951 | 1:500 | NA |
Dextran-FITC 150 kDa | Sigma Aldrich | 46946 | 10 mg/mL | NA |
