Fig. 6: Human gastric organoid/macrophage co-cultures.
From: Sonic Hedgehog acts as a macrophage chemoattractant during regeneration of the gastric epithelium
A Acridine orange assay using gastric organoids treated with either vehicle or histamine. B Shift in F458 (red)/F488 (green) fluorescence ratio was measured in the organoid cultures treated with vehicle or histamine. C Quantitative RT-PCR measuring changes in Shh expression in the gastric organoids treated with vehicle (control) or histamine (Hist). *P < 0.05 compared to control, n = 4 cultures generated from four individual patients. D Bright-field micrographs showing tissue-derived human fundic gastric organoids (huFGOs), PBMC-derived macrophages, and representative co-culture. E Magnetic separation was used to isolate epithelial and immune cells from treated human gastric organoid/macrophage co-cultures. F RT-PCR demonstrating expression of Shh in EpCAM positive and CD68 in macrophage fractions. Data are shown as the mean ± SEM.
