Fig. 4: AAT scaffolds skew macrophages towards an M2-phenotype in muscle wounds. | npj Regenerative Medicine

Fig. 4: AAT scaffolds skew macrophages towards an M2-phenotype in muscle wounds.

From: An immunologically active, adipose-derived extracellular matrix biomaterial for soft tissue reconstruction: concept to clinical trial

Fig. 4

a CD206 and CD86 expression in macrophages (CD45+SiglecF-CD11b+CD3-Ly6g−Ly6c−F4/80+) isolated from healthy quadriceps muscle and scaffold- or saline-treated muscle wounds after 1-week post-op. b Populations of alternately activated “M2” (CD206+CD86−), classically activated “M1” (CD206−CD86+), double-polarized (CD206+CD86+) and nonpolarized (CD206−CD86−) macrophages quantified by flow cytometry. c Expression of canonical M2 (Il4, Arg1) and M1 (Ifng, Nos2) genes at the wound site by qRT-PCR. Significance relative to vehicle control is depicted (p-values), see Supplementary Table 2 for other significance values: * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001. Error bars represent ±1 standard deviation.

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