Fig. 3: Early DNA methylation inhibition reduces BEC dedifferentiation through p53 activation.
From: DNA methylation maintenance at the p53 locus initiates biliary-mediated liver regeneration
a Experimental scheme illustrating the 5azaC and Mtz treatment and analysis from R0h to R48h. b Bisulfite sequencing analysis of DNA methylation at CpG islands near the promoter at the p53 locus in DNA isolated from DMSO and 5azaC-treated livers at BT and R0h. c WISH images showing the expression of p53 during liver regeneration after early DNA methylation inhibition. Quantification of the intensity of p53 expression in liver regions from R0h to R48h. d Single-optical section images showing the expressions of hepatoblast markers hhex, foxa3, sox9b, and mTORC1 effectors pS6 and p4EBP1 at R8h in WT and p53 mutant after early DNA methylation inhibition. Note that the expressions of hepatoblast markers and mTORC1 signaling were rescued in p53 mutant compared with WT after early DNA methylation inhibition. Asterisks indicate statistical significance: ****P < 0.0001 using t-tests analysis when compared to control. Numbers indicate the proportion of larvae exhibiting the expression shown. Scale bars: 100 µm; error bars, ±SEM. DAPI 4′, 6-diamidino-2-phenylindole, BT before treatment, FISH fluorescent in situ hybridization, R regeneration time after the withdrawal of Mtz.
