Fig. 4: Loss of p53 rescues liver regeneration defect after early DNA methylation inhibition.
From: DNA methylation maintenance at the p53 locus initiates biliary-mediated liver regeneration
a Experimental scheme illustrating the 5azaC and Mtz treatment and analysis at R48h. b WISH images showing the expressions of hepatocyte markers cp and gc at R48h in WT and p53 mutant after early DNA methylation inhibition. Note that the expressions of cp and gc were recovered in p53 mutant after 5azaC treatment. Quantification of the intensity of cp and gc expression in liver regions at R48h. c Single-optical section images showing the expressions of hepatocyte marker Bhmt and BECs marker Alcam in WT and p53 mutant after early DNA methylation inhibition and controls at R48h. Quantification of the intensity of Bhmt expression and the percentage of Alcam+ among Dendra2+ cells in liver regions at R48h. Note that the expression of Bhmt was recovered in p53 mutant compared to WT after 5azaC treatment. Asterisks indicate statistical significance: ***P < 0.001; ****P < 0.0001 using t-tests analysis when compared to control. Scale bars: 100 µm; error bars, ±SEM. DAPI 4′, 6-diamidino-2-phenylindole, BT before treatment, ns no significant difference, R regeneration time after the withdrawal of Mtz.
