Fig. 7: The dnmt1 mutant blocks liver regeneration.

a Single-optical section images showing the expression of pS6, p4EBP1, and Anxa4 in regenerating livers at R0h in dnmt1 mutant and sibling. Note that the mTORC1 signaling effectors pS6 and p4EBP1 are downregulated in dnmt1 mutant compared with the sibling. b Single-optical section images showing the FISH and antibody staining images of the expressions of hhex, sox9b, foxa3, and Anxa4 in regenerating livers at R0h in dnmt1 mutant and sibling. c Bisulfite sequencing analysis of DNA methylation at the p53 locus in DNA isolated from livers of dnmt1 sibling and mutant at R0h. d qPCR data showing the relative expression level of p53 in livers of dnmt1 mutant and sibling at R0h. e WISH images showing the expressions of cp and gc at R48h in dnmt1 mutant and sibling livers (arrows). Quantification of the intensity of gc and cp expressions in liver regions at R48h. f Single-optical section images showing the expressions of hepatocyte marker Bhmt, BECs marker Alcam, and Dendra2 in regenerating livers at R48h in dnmt1 mutant and sibling. g WISH images showing the expression of p53 at R0h, R8h, R24h, and R48h in dnmt1 mutant and sibling livers (arrows). Asterisks indicate statistical significance: ***P < 0.001; ****P < 0.0001 using t-tests analysis when compared to control. Numbers indicate the proportion of larvae exhibiting the expression shown. Scale bars: 100 µm; error bars, ±SEM. DAPI 4’,6-diamidino-2-phenylindole, FISH fluorescent in situ hybridization, R regeneration time after the withdrawal of Mtz.