Fig. 5: Pai1−/− mice demonstrate increased wound healing post-injury.
From: Proteoglycan 4 (PRG4) treatment enhances wound closure and tissue regeneration
Pai1−/− mice demonstrate increased wound closure compared to C57BL/6 mice and this is enhanced in the presence of rhPRG4 a. Representative images of ears 4 weeks post-injury b, c. The black dashed line represents the plane of histological sectioning which the original injury diameter, while the white dashed box indicates the location of the histological image presented b, c. Representative histological images stained with Safranin O, 4 weeks post-injury d, e. Minimal new cartilage can be observed in Pai1−/− mice treated with DMSO (arrows, d). Although the wound completely closed in Pai1−/− mice treated with rhPRG4, new cartilage is absent from the wound area (arrow e) yet is present at the injury boundary. Quantitative analysis of blood flow data demonstrates that Pai1−/− mice treated with DMSO have no increase in blood flow at the wound area post-injury f. Furthermore, the number of CD31 + blood vessels is decreased in the wound area site of Pai1−/− mice g. Scale bar equals 50 µm. n.s. = not significant. Error bars equal mean ± SD (f, g – 1 way ANNOVA, a – 2 way ANNOVA). Sample sizes: a-f, n = 15 per group; g–i, n = 6 per group.
