Table 8 Constraints in experimental design or system optimization in cell culture and tissue engineering.
Constraint class | Examples of metrics to assess in optimization scheme |
|---|---|
Cost | Culture medium |
Culture device | |
Cell source | |
Reliability | Batch variability |
Cell line variability | |
Predictivity | Sensitivity to differentiate disease |
Sensitivity to differentiate toxicity | |
User | Time requirement to use |
Skill requirement to use | |
Time | Lead time to prepare experiment |
Culture time to measurement | |
Biological function of cell/tissue | Catalysis |
Secretion | |
Electrophysiology | |
Barrier function | |
Perfusion | |
Contractility | |
Morphology | |
Transcription | |
Protein expression |
