Fig. 2: Engraftment is persistent even in the context of bursts of DUX4 expression.
From: Transplantation of PSC-derived myogenic progenitors counteracts disease phenotypes in FSHD mice
a Outline of experimental design. b Representative images, capturing the complete engrafted area, show immunostaining for RFP (in red), Dys (in gray), and DAPI (in blue) of the 7 + 7 cohort. Scale bar is 200 µm. c Representative images show the same staining as b for the 3 experimental groups depicted in a: 7 + 0, 7 + 7, and 7 + 14. Upper panel shows PBS-injected control. Scale bar is 50 µm. d Graph shows quantification of engraftment (from c) as shown by the number of donor-derived RFP + /Dys+ myofibers. Data are shown as mean ± SEM (n = 7 for PBS, n = 13 for 7 + 0, n = 18 for 7 + 7 and n = 6 for 7 + 14). **p < 0.01, ***p < 0.001 by the Student’s t test. e Distribution of the number of RFP + /Dys+ myofibers along the TA muscle. Data are shown as mean ± SEM (n = 13 for 7 + 0, n = 18 for 7 + 7, and n = 6 for 7 + 14).
