Fig. 3: Testing environment vs. cell autonomous effect of dox post-transplant.
From: Transplantation of PSC-derived myogenic progenitors counteracts disease phenotypes in FSHD mice
a Representative images show immunostaining for RFP (in red) and Dys (in gray) in TA muscles of BL6 mice that had been injected with PBS or Pax3-induced myogenic progenitors, and later fed with dox in a similar fashion as iDUX4pA-HSA 7 + 0 and 7 + 14 cohorts (Fig. 2a). DAPI stained nuclei in blue. Scale bar is 50 µm. b Graph shows engraftment quantification from a. Data are shown as mean ± SEM (n = 7 for 7 + 0 and n = 7 for 7 + 14). c Representative images show immunostaining for tdTomato (in red), and Dys (in gray) in TA muscles of iDUX4pA-HSA mice that had been injected with PBS or 5000 satellite cells isolated from a mTmG mouse and fed with dox following the 7 + 0 and 7 + 14 dox regimen. DAPI stained nuclei in blue. Scale bar is 50 µm. d Graph shows engraftment quantification from c based on the number tdTomato + /Dys+ myofibers. Data are shown as mean ± SEM (n = 6 for 7 + 0 and n = 5 for 7 + 14). p = 0.061 by the Student’s t test.
