Fig. 4: Transplantation of myogenic progenitors counteracts the fibrosis induced by DUX4 expression in iDUX4pA-HSA mice.
From: Transplantation of PSC-derived myogenic progenitors counteracts disease phenotypes in FSHD mice
a Representative images show Masson’s trichrome staining for the 3 experimental cohorts of iDUX4pA-HSA mice: 7 + 0, 7 + 7, and 7 + 14. Right panels show transplanted TA muscles, while left panels display respective contralateral PBS controls. Scale bar is 100 µm. b Quantification of the percentage of collagen staining in the total muscle section. Data are shown as mean ± SEM (n = 13 for 7 + 0, n = 18 for 7 + 7 and n = 6 for 7 + 14). *p < 0.05 by the Student’s t test. c Representative images of a whole muscle section show immunostaining for RFP (in red), Lam (in green), and Col VI (in purple; staining performed on the consecutive slide) in transplanted TA muscle from iDUX4pA-HSA mice (7 + 14). Engrafted area has been highlighted in red to denote the engraftment location on the Col VI staining. DAPI in blue. Scale bar is 500 µm. d Images show higher magnification of negative and positive RFP areas from c (from white dashed lines used to depict RFP- and RFP + areas). Scale bar is 50 µm.
