Fig. 2: Enhancement of neuronal morphology after patterning by BMP inhibition.

a Timeline of LDN193189 and/or dox treatments of POU4F2-tdTomato PSCs with an integrated empty cassette (control) or NAIP2 cassette (conditions 1–3). Brightfield images of dox treated b empty cassette control cells, c NAIP2-nc, d NEUROG2, e POU4F2, f ATOH7, and g ISLET1 cells after 6 days. NAIP2-nc = not clonally selected. h Quantification of %POU4F2+ cells at day 6 relative to DAPI in the empty cassette control and conditions 1–3 illustrated in (a). *P < 0.05, **P < 0.01, ***P < 0.001, ns P > 0.05, n = 3. Error bars are reported as standard deviation (SD). i–n NAIP2 RGC-iNs from different genetic backgrounds differentiated and imaged in brightfield and POU4F2+ tdTomato fluorescence for clonally selected i, j IMR90.4 and k, l GM23720 PSCs, and m, n brightfield and POU4F2 + h2b-mNeonGreen fluorescence for WA09 PSCs. Click-iT EdU staining in o undifferentiated PSCs and 2-day LDN/dox treated p control, q NEUROG2, and r NAIP2 cells co-stained with DAPI. Arrows in O-R indicate EdU+ cells. Scale b–g, i–n = 100 µm, o–r = 200 µm.