Fig. 2: The TM regenerates without permanent scarring.
From: Epimorphic regeneration in the mammalian tympanic membrane
a RNAscope showing expression of Krt5 (red) and Krt10 (green) in a cross-section of the TM from day 7 post-injury, illustrating transient mixed stratification within the epidermis. The yellow magnified region highlights an area directly over the wound site. White arrows denote cells co-expressing Krt5 and Krt10. b Immunofluorescence (IF) for Pdgfra in a TM cross-section from day 7, demonstrating the newly formed multi-lineage stratification of the TM. c IF for Krt5, Krt23, and Flg in TM cross-sections from day 7 and day 14 post-injury, demonstrating expansion and quick dissipation of cells positive for these markers. d IF for COLII (green) in representative whole-mount TMs harvested at the indicated timepoints post-injury reveals restoration of normal collagen patterning of the TM by year 1 (n = 5 mice per timepoint, except n = 3 for 1 year). Scale bars: 100 μm.
