Fig. 4: Src, Map4k4 and p38 MAPK are indispensable for CXCR2 function.
From: Implication of CXCR2-Src axis in the angiogenic and osteogenic effects of FP-TEB
a Representative images of migrated HUVECs in Transwell systems. Quantification of the migrated HUVECs is shown as a bar graph (n = 10). Scale bars,100 μm. b Representative images of wound healing assays. The rate of scratch wound closure is shown as a bar graph (n = 10). Scale bars, 200 μm. c Schematic diagram of the experiments for assessing the angiogenic and osteogenic effects. d Representative immunostaining images with quantification of cell numbers in FP-TEBs implanted into bone defects of cxcr2flox mice, which at 2 weeks postoperatively (n = 5). White arrows, staining-positive cells. Scale bar, 10 μm. e Representative Micro-fill perfusion images of bone defect areas from cxcr2flox mice treated with FP-TEBs at 2 weeks postoperatively. f H&E and Masson staining at 4 week postoperateively. Dotted straight line indicates the femur–implant junction. Scale bars 500 μm. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01.
