Fig. 2: Induction of senescence driven injury in Mdm2 mice by AAV8.TBG.Cre results in time sensitive changes in endothelial cell activation, myofibroblast activation and collagen-I deposition. | npj Regenerative Medicine

Fig. 2: Induction of senescence driven injury in Mdm2 mice by AAV8.TBG.Cre results in time sensitive changes in endothelial cell activation, myofibroblast activation and collagen-I deposition.

From: Utilising an in silico model to predict outcomes in senescence-driven acute liver injury

Fig. 2

Purple dashed lines are healthy control mean ± SEM. Data points represent mean of N = 3–5 independent animals per timepoint analysed. All error bars are ±SEM. All qPCR results were normalised to the PPIA housekeeper and expressed as fold change relative to healthy control mean expression. For histological staining quantification of the % of the field of view (FOV) positively stained was assessed unless otherwise indicated, with ≥10 FOVs analysed per animal. a Representative immunofluorescent micrographs showing co-staining of the endothelial marker, ERG and activated endothelial cell marker VCAM-1 in healthy control animals and at D3, D7 and D14 following senescence induction, alongside quantification of ERG+VCAM-1+ cells. Results show a time sensitive increase in dual-positive endothelial cells indicating a time sensitive increase in this activated cell population, with expression resolving to healthy control animal levels by D14. Scale bars 100 µm (white) and 20 µm (green). b Fold change in total gene expression of the activated endothelial cell markers VCAM-1, ICAM-1 and ACKR3 following senescence induction. c Representative histological micrographs and quantification showing the time sensitive increase in staining for the activated myofibroblast marker α-SMA. Scale bars 100 µm. d Analysis of changes in gene expression of the activated myofibroblast markers α-SMA and PDGFRB over time by qPCR. e Representative histological micrographs and quantification showing the time sensitive increase in staining for collagen-I. Scale bars 100 µm. f Analysis of changes in gene expression of the pro-alpha1 and pro-alpha2 chains of collagen-I (COL1A1 and COL1A2) over time by qPCR.

Back to article page