Fig. 2: Cellular uptake mechanisms of QDP/small RNA. | npj Regenerative Medicine

Fig. 2: Cellular uptake mechanisms of QDP/small RNA.

From: Quantum dots as biocompatible small RNA nanocarriers modulating macrophage polarization to treat Asherman’s syndrome

Fig. 2

A, B FACS histograms represent the uptake efficiency of C-QDs and QDP/FAM-small RNA at 37 °C and 4 °C incubation. The graph shows the average uptake efficiency of C-QDs at both temperatures. C, D FACS histograms represent the uptake efficiency of C-QDs and QDP/FAM small RNA in the presence of various endocytosis inhibitors. The graph shows the average uptake efficiency of C-QDs in the presence of various inhibitors. Each bar represents the mean ± SD of three independent experiments. Statistical analyses were performed using a t-test. *p < 0.05, **p < 0.01. E Confocal images represent immunofluorescence staining for EEA1 (early endosome marker) and LAMP-1 (late endosome marker) in QDP/FAM-small RNA-treated cells. White arrowheads indicate the co-localization of the QDP and endosomes. Yellow arrowheads indicate the QDP escaped from the endosomes. Green arrowheads indicate free FAM-small RNA released into the cytoplasm. Nucleus (Blue), QD (Red), FAM-small RNA (Green), Endosome (Magenta). The scale bar represents 10 μm.

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