Fig. 5: Unbiased LC-MS/MS-based single-cell proteomic profiling of purified primitive salivary cells. | npj Regenerative Medicine

Fig. 5: Unbiased LC-MS/MS-based single-cell proteomic profiling of purified primitive salivary cells.

From: Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1

Fig. 5

A Overall workflow for single cell proteomics. Single cells (P1) from PG and SMG cells were isolated by FACS using DAPI-CD45-CD31-EpCAM+ immunophenotype, lysed and digested in 384-well plate using cellenONE platform. Mass spectrometry data of each single cell were acquired in diaPASEF mode using the timsTOF SCP mass spectrometer. A total of 2461 proteins across 271 single cells were identified (created using Biorender.com). B Number of identified proteins per cell from SMG and PG P1 cultures. On average, 1143 proteins and 1270 proteins per single cell were identified from PG and SMG, respectively. C A Venn diagram showing number of shared and unique differentially expressed proteins within the progenitor-rich single cells of both SMG and PG. D Dot plot showing protein counts per cell of 20 most abundantly detected proteins in progenitor rich cells in total salivary gland, SMG and PG. E Pie chart showing the sub-cellular location of proteins detected in PG (N = 2, n = 142) and SMG cells (N = 2, n = 129). G Pie chart showing the molecular type of proteins detected in PG (N = 2, n = 142) and SMG cells (N = 2, n = 129). N represents the number of patient samples and n represents number of SGSPC analyzed. Ingenuity pathway analysis was used for categorizing subcellular localization and function of proteins (QIAGEN Inc., https://digitalinsights.qiagen.com/IPA). N denotes the number of patients; n denotes the number of single cells.

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