Fig. 6: Unbiased single cell proteome profiling identifies salivary stem/progenitor-specific cytokeratin profile. | npj Regenerative Medicine

Fig. 6: Unbiased single cell proteome profiling identifies salivary stem/progenitor-specific cytokeratin profile.

From: Establishment of salivary tissue-organoid biorepository: characterizing salivary gland stem/progenitor cells and novel differentiation marker PSMA/FOLH1

Fig. 6

A A plot showing the relative abundance of cytokeratins detected per individual cells (P1) from PG and SMG cells isolated by FACS using DAPI-CD45-CD31-EpCAM+ immunophenotype enriching for SGSPC activity. B The relationship between the keratin expression profile identified through single-cell proteomics in SGSPC and the salivary epithelial cell-type specific expression patterns in the immunohistochemistry-based map of human protein expression profiles explored through the Human Protein Atlas (https://www.proteinatlas.org/). C SGSPC-expressed keratins map to scattered ductal basal cells in salivary glands (Source: Human Protein Atlas, https://www.proteinatlas.org/). D UMAP-based cluster analysis of mouse SMG scRNA-seq data showing salivary cell clusters. E Dot plot showing the expression of murine analogs of human SGSPC-expressed cytokeratins (and EpCAM) across mouse salivary cell types (*, ** *** indicate p-values < 0.05, 0.005 0.0005, and 0.0001 respectively).

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